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Title Evaluation of the Embryotoxic and Teratogenic Effects of Decalobanthus peltatus (Buwakan) Leaf Extract on Duck Embryo
Posted by Mylah Tabelin
Authors Merrah Joy B. Subebe, Josephine C. Ogaro, Melanie Dayon, Michael Muhmin Manting, Aki Murashima, Akiko Omori, Jaime Q. Guihawan, Mylene M. Uy Ahmad Reza Mazahery, Mylah Villacorte-Tabelin
Publication date N/A
Conference 56th Annual Meeting of Japanese Society of Developmental Biologist
Abstract Decalobanthus peltatus is a woody vine that is commonly utilized in traditional Southeast Asian medicinal preparations. The leaves are generally used as herbal remedy with a broader range of pharmacological activity. Despite the well documented therapeutic uses of D. peltatus, there is currently little information on its toxicity. The current study aimed to assess the embryotoxicity and teratogenic effects following crude leaf extracts of D. peltatus (Ethanol, ethyl acetate, and n-hexane) treatment on duck embryos. Brine shrimp lethality assay was used to determine preliminary cytotoxicity of the crude leaf extracts. Ethyl acetate and hexane extracts were considered highly toxic with an LC50 of 14.54 ppm and 26.30 ppm, respectively. Based on the brine shrimp lethality assay, the duck embryos were exposed to the leaf extract with the lowest LC50 with concentrations of 1000 ppm and 500 ppm. Here, the alteration of vascular branching pattern in the duck’s chorioallantoic membrane and gross morphological and histopathological effects of the leaf extract on the skin were observed and quantified. Results of the study showed decreased vessel junctions and vessel density in both concentrations. Embryo malformations, growth retardation and hemorrhages on the external body surfaces were observed in duck embryos treated with concentration of 1000 ppm. Histopathological analysis of the skin at 14-day-old duck embryos showed reduced number of feather follicles as compared to control. Overall, the findings demonstrated that D. peltatus may present risks when taken in significant dosages, particularly for pregnant mothers. As a result, comprehensive toxicity testing on therapeutic herbs should be performed to ensure their safety on the embryos and their development.
Index terms / Keywords CAM assay, Decalobanthus peltatus, embryo malformations, embryotoxic, medicinal plants, teratogenic