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Title First report of Caenorhabditis brenneri (Nematoda: Rhabditida) isolated from the cadaver of Philippinella moellendorffi (Stylommatophora: Ariophantidae), a terrestrial slug in the Philippines
Posted by Nanette Hope Sumaya
Authors Loel Dalan, Michelle Anne Diano, Irma Tandingan De Ley & Nanette Hope Sumaya
Publication date 2022/08/04
Journal Journal of Helminthology
Volume 96
Issue e59
Pages 1-5
Publisher Cambridge University Press
Abstract Gastropod-associated nematodes have been previously studied and documented worldwide, with some species forming host-specific association as obligate parasites of molluscs while others form intermediate and temporary association. Philippinella moellendorffi from Imelda, Zamboanga Sibugay, Philippines, were collected, washed and maintained in the laboratory until death. Cadavers were placed on nutrient agar to allow nematode proliferation. Nematode pure culture was obtained using one gravid female for propagation. Morphology and molecular analyses (18S ribosomal DNA (rDNA) and D2–D3 expansion segments of 28S rDNA) were employed as diagnostic tools in identifying the nematode species isolated from P. moellendorffi. The newly isolated nematode was identified as Caenorhabditis brenneri, thus designated as C. brenneri strain IZSP from the Philippines. This is the first record of C. brenneri isolated from the terrestrial slug P. moellendorffi.
Index terms / Keywords Caenorhabditis; gastropods; Philippinella; 18S rDNA; D2–D3 expansion segments of 28S rDNA
DOI S0022149X22000475