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Title Isolation and biocontrol potential of entomopathogenic and entomophilic nematodes from Talakag, Bukidnon and Claveria, Misamis Oriental, Philippines
Posted by Nanette Hope Sumaya
Authors Nanette Hope Sumaya, Cynthia Ann Dichusa, Mary Flor Maureal, Jill Kate Lluisma, Janna Andalan
Publication date 2022/05/20
Conference 7th International Congress of Nematology 2022
Volume Book of Abstracts
Issue Online
Pages 673
Publisher Alphavisa
Abstract Amidst rising concerns over rampant synthetic chemical applications and their deleterious effects, the identification of alternative biopesticides has become paramount. Entomopathogenic and entomophilic nematodes (EPNs) of the families Steinernematidae, Heterorhabditidae and Rhabditidae are used as biocontrol agents (BCAs) against several insect pests. In this study, local nematodes as BCAs were explored in different agricultural areas of Talakag, Bukidnon and Claveria, Misamis Oriental. Insect-baiting using the larvae of the superworm, Zophobas morio and greater wax moth, Galleria mellonella was carried out. Out of 70 soil samples collected, only 10 samples harbored nematodes from the different sampling areas in Talakag (carrot, cabbage, broccoli, bell pepper, maize, mungbean and potato) and in Claveria (cabbage, eggplant, chayote, cassava, dragon fruit, maize and pineapple). Through morphology and analyses of ITS and SSU rDNA regions, we identified and confirmed nematode isolates belonging to family Rhabditidae: Oscheius and Metarhabditis spp. from Talakag and Claveria. Unexpectedly, the widely recognized EPNs Heterorhabditis and Steinernema were not recovered in this study. Furthermore, the efficacy of the local nematode isolates from Talakag was assessed against the last instar larvae of pineapple white grub, Anomala orientalis under laboratory conditions. At 48 hours post inoculation, the mean mortality induced by O. carolinensis, O. colombiana and M. amsactae were 100%, 100% and 55.6%, respectively. For nematode isolates from Claveria, Oscheius sp. and Metarhabditis sp., the efficacy will be tested against the cotton cutworm, Spodoptera litura and then compared with the commercially available Steinernema siamkayai. Our findings highlight the potential of locally-occurring nematodes to be utilized as BCAs of agricultural target pests after further comprehensive trials.
Index terms / Keywords Biocontrol - Morphology - Molecular characterization - Anomala - Efficacy