Journal
| Title | High-throughput sequencing-based analysis of bacterial communities associated with Barbour's seahorses (Hippocampus barbouri) from Surigao del Norte, Philippines Posted by Sharon Rose Tabugo |
| Authors | Rose Chinly Mae H. Ortega, Sharon Rose M. Tabugo, Joey Genevieve T. Martinez, Chinee S. Padasas, Marilen P. Balolong, José L. Balcázar |
| Publication date | 2021 |
| Journal | Letters in Applied Microbiology |
| Pages | 1-6 |
| Publisher | WILEY , 111 RIVER ST, HOBOKEN, USA, NJ, 07030-5774 |
| Abstract | This study identified the bacterial community composition associated with BarbourâÂÂs seahorses (Hippocampus barbouri). Seahorses and samples from the surrounding environment (sediment and water) were collected from Cantiasay Island in Surigao del Norte, Philippines. Genomic DNA was isolated from these samples, and the V1âÂÂV3 region of the 16S rRNA gene was amplified and sequenced on the Illumina MiSeq platform. There were 929 803 sequence reads corresponding to 859 operational taxonomic units (OTUs), which were obtained from the six genomic libraries. The results showed that the most abundant OTUs were affiliated to the phylum Proteobacteria, particularly those belonging to the genera Shewanella, Pseudomonas, Acinetobacter and Aeromonas, which were found to be dominant in seahorse gut samples. The only exception was for the skin of male seahorses, which was dominated by members belonging to the genus Bacillus (phylum Firmicutes). These findings on bacterial community structure and composition may shed light on therapeutic claims of BarbourâÂÂs seahorses and help in the conservation of this species. |
| Index terms / Keywords | bacterial community composition, Hippocampus barbouri, seahorses. |
| DOI | doi:10.1111/lam.13511 |
| URL | https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/lam.13511 |