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Title The Venom of Philippine Tarantula (Theraphosidae) Contains Peptides with Pro-Oxidative and Nitrosative-Dependent Cytotoxic Activities against Breast Cancer Cells (MCF-7) In Vitro
Posted by Olga Nueza
Authors Simon Miguel M Lopez1, Jeremey S Aguilar1, Jerene Bashia B Fernandez1, Angelic Gayle J Lao1, Mitzi Rain R Estrella1, Mark Kevin P Devanadera1,2, Anna Beatriz R Mayor2,3, Leonardo A Guevarra Jr1,2, Myla R Santiago-Bautista1,2,3*, Olga M Nuñeza4, Librado A Santiago1,2,3
Publication date 2020/08
Journal Asian Pacific Journal of Cancer Prevention
Volume 21
Issue 8
Pages 2423-2430
Publisher Asian pacific organisation for cancer prevention
Abstract Background: Breast cancer is a multifactorial disease that affects women worldwide. Its progression is likely to be executed by oxidative stress wherein elevated levels of reactive oxygen and nitrogen species drive several breast cancer pathologies. Spider venom contains various pharmacological peptides which exhibit selective activity to abnormal expression of ion channels on cancer cell surface which can confer potent anti-cancer activities against this disease. Methods: Venom was extracted from a Philippine tarantula by electrostimulation and fractionated by reverse phase-high performance liquid chromatography (RP-HPLC). Venom fractions were collected and used for in vitro analyses such as cellular toxicity, morphological assessment, and oxidative stress levels. Results: The fractionation of crude spider venom generated several peaks which were predominantly detected spectrophotometrically and colorimetrically as peptides. Treatment of MCF-7 cell line of selected spider venom peptides induced production of several endogenous radicals such as hydroxyl radicals (•OH), nitric oxide radicals (•NO), superoxide anion radicals (•O2−) and lipid peroxides via malondialdehyde (MDA) reaction, which is comparable with the scavenging effects afforded by 400 μg/mL vitamin E and L-cysteine (p<0.05). Concomitantly, the free radicals produced decrease the mitochondrial membrane potential and metabolic activity as detected by rhodamine 123 and tetrazolium dye respectively (p>0.05). This is manifested by cytotoxicity in MCF-7 cells as seen by increase in membrane blebbing, cellular detachment, caspase activity and nuclear fragmentation. Conclusion: These data suggest that the Philippine tarantula venom contains peptide constituents exhibiting pro-oxidative and nitrosative-dependent cytotoxic activities against MCF-7 cells and can indicate mechanistic insights to further explore its potential application as prooxidants in cancer therapy.
Index terms / Keywords Philippine tarantula- spider venom- cytotoxicity- MCF-7- oxidative stress10
DOI 10.22034/APJCP.2017