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Title An ex vivo culture system to study thyroid development
Posted by Mylah Tabelin
Authors Anne-Sophie Delmarcelle, Mylah Villacorte, Anne-Christine Hick, Christophe E Pierreux
Publication date 2014/6/6
Journal JoVE (Journal of Visualized Experiments)
Issue 88
Pages e51641
Publisher JoVE
Abstract This protocol describes dissection of mouse embryonic thyroid anlagen and the culture of explants on semiporous filters or on microscopy plastic slides. This system is ideal to study morphogenetic or differentiation events occurring during thyroid development of wild type or knockout embryos, and is amenable to gain- and loss-of-function experiments.
Index terms / Keywords thyroid, explants, anlagen
DOI doi: 10.3791/51641