Journal
| Title | Anti-PirA-like toxin immunoglobulin (IgY) in feeds passively immunizes shrimp against acute hepatopancreatic necrosis disease Posted by Ivane Gerasmio |
| Authors | Pedrosa-Gerasmio, Ivane R, Nakamura, Rika; Alenton, Rod Russel R; Kondo, Hidehiro; Hirono, Ikuo |
| Publication date | 2019/05/21 |
| Journal | Journal of Fish Diseases |
| Volume | 42 |
| Issue | 8 |
| Pages | Pages 1125-1132 |
| Publisher | Wiley |
| Abstract | Acute hepatopancreatic necrosis disease (AHPND), caused by a toxinâÂÂproducing Vibrio parahaemolyticus strain, has become a serious threat to shrimp aquaculture. The need to regulate antibiotic use prompted the development of alternative ways to treat infections in aquaculture including the use of chicken egg yolk immunoglobulin (IgY) for passive immunization. This study evaluated the protective effect of IgY against AHPND infection in Litopenaeus vannamei (Boone). IgY was isolated from eggs laid by hens immunized with recombinant PirAâÂÂlike (rPirA) and PirBâÂÂlike (rPirB) toxins. WholeâÂÂegg powders having IgY specific to rPirA (antiâÂÂPirAâÂÂIgY) and rPirB (antiâÂÂPirBâÂÂIgY) and IgY from nonâÂÂimmunized hen (controlâÂÂIgY) were mixed with basal diets at 20% concentrations and used to prefeed shrimp 3 days before the bacterial challenge test. Survival rates of the challenged shrimp fed the antiâÂÂPirAâÂÂIgY, antiâÂÂPirBâÂÂIgY and controlâÂÂIgY diets were 86%, 14% and 0%, respectively. Only the feed containing antiâÂÂPirAâÂÂIgY protected shrimp against AHPND. Increasing the concentration of rPirA antigen to immunize hens and lowering the amount of egg powder in feeds to 10% consistently showed higher survival rates in shrimp fed with antiâÂÂPirAâÂÂIgY (87%) compared with the control (12%). These results confirm that addition of antiâÂÂPirAâÂÂIgY in feeds could be an effective prophylactic method against AHPND infection in shrimp. |
| Index terms / Keywords | acute hepatopancreatic necrosis disease, egg yolk immunoglobulin, Litopenaeus vannamei, Vibrio parahaemolyticus |
| DOI | https://doi.org/10.1111/jfd.13024 |
| URL | https://onlinelibrary.wiley.com/doi/abs/10.1111/jfd.13024 |