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Title Gut bacterial community profile in Pacific white shrimp Litopenaeus vannamei following 5-aminolevulinic acid supplementation
Posted by Ivane Gerasmio
Authors Pedrosa-Gerasmio Ivane R; Nozaki Reiko; Kondo, Hidehiro; Hirono, Ikuo
Publication date 2020/06
Journal Aquaculture Research
Volume 00
Pages 1-12
Publisher Wiley
Abstract Changes in the diet have been previously reported to alter the gut bacterial profile in several organisms, including shrimp. These shifts in microbial structure either promote beneficial effects to the host or cause diseases. Supplementation of 5-aminolevulinic acid (5-ALA), the precursor of tetrapyrroles, has been previously reported to enhance shrimp's immune response. To know whether 5-ALA has effects on the gut bacterial structure in Pacific white shrimp (Litopenaeus vannamei), we investigated the bacterial communities in the intestine and stomach of L. vannamei using highthroughput Illumina sequencing of 16S rRNA gene libraries. One week of 5-ALA supplementation altered the bacterial community structure (beta diversity) in both tissues, as shown by the results of multi-dimensional scaling (MDS) plots and analysis of similarities (ANOSIM). DESeq2 analysis revealed enrichment of differentially abundant taxa in the 5-ALA group (e.g. Enhydrobacter and Oceaniovalibus) and control group (e.g. Tenacibaculum and Mycobacterium). Metagenomic predictions suggested that the control group had more KEGG pathways associated with ‘metabolism’ than the 5-ALA group. This study suggests that 5-ALA supplementation potentially promotes the formation of a beneficial bacterial community structure in shrimp. This is the first report on the effect of 5-ALA supplementation on the bacterial community profile in any organism.
Index terms / Keywords 5-aminolevulinic acid, 16S rRNA gene, gut microbiome, high-throughput sequencing, Litopenaeus vannamei