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Title Physical properties of the extracellular matrix of decellularized porcine liver
Posted by Ronald Bual
Authors Ijima, Hiroyuki; Nakamura, Shintaro; Bual, Ronald; Shirakigawa, Nana; Tanoue, Shuichi
Publication date 2018/6
Journal Gels
Volume 4
Issue 2
Pages 39
Publisher Multidisciplinary Digital Publishing Institute
Abstract The decellularization of organs has attracted attention as a new functional methodology for regenerative medicine based on tissue engineering. In previous work we developed an L-ECM (Extracellular Matrix) as a substrate-solubilized decellularized liver and demonstrated its effectiveness as a substrate for culturing and transplantation. Importantly, the physical properties of the substrate constitute important factors that control cell behavior. In this study, we aimed to quantify the physical properties of L-ECM and L-ECM gels. L-ECM was prepared as a liver-specific matrix substrate from solubilized decellularized porcine liver. In comparison to type I collagen, L-ECM yielded a lower elasticity and exhibited an abrupt decrease in its elastic modulus at 37 °C. Its elastic modulus increased at increased temperatures, and the storage elastic modulus value never fell below the loss modulus value. An increase in the gel concentration of L-ECM resulted in a decrease in the biodegradation rate and in an increase in mechanical strength. The reported properties of L-ECM gel (10 mg/mL) were equivalent to those of collagen gel (3 mg/mL), which is commonly used in regenerative medicine and gel cultures. Based on reported findings, the physical properties of the novel functional substrate for culturing and regenerative medicine L-ECM were quantified.
Index terms / Keywords liver-specific extracellular matrix; gel; scaffold; tissue engineering; decellularization; solubilized extracellular matrix; physical property; storage modulus; loss modulus; biodegradation