|Title||Cytotoxicity of 2-Geranylgeranyl-1,4-hydroquinone Isolated from Philippine Marine Sponge Carteriospongia sp.
Posted by Chona Gelani
|Authors||Chona D. Gelani, Mylene M. Uy, Shinji Ohta, Emi Ohta|
|Conference||98th Annual Meeting of the Chemical Society of Japan|
|Abstract||Bioassay-guided purification of the hexane:ethyl acetate extract of the Philippine marine sponge, Carteriospongia sp. , resulted in the isolation of a bioactive substance which inhibited the embryonic development of sea urchin (Hemicentrotus pulcherrimus) and starfish (Asterina pectinifera) at the early gastrulation stage at a minimum concentration of 6.25 mcg/mL and 12.5 mcg/mL, respectively. (+)HR-ESI-FTMS gave an [M+Na]+ peak corresponding to m/z 405.2763 which confirms the structure with the molecular weight 382.2865. The 1 H and 13C NMR spectra of the bioactive compound were identical to those of 2-geranylgeranyl-1,4-hydroquinone, a known cytotoxic metabolite from a southern Australian deep-water marine sponge Fasciospongia sp. As of this writing, no study has been published yet regarding the inhibitory effects of 2-geranylgeranyl-1,4-hydroquinone on the embryonic development of sea urchin and starfish. When subjected to MTT assay, 2-geranylgeranyl-1,4-hydroquinone was found to be low cytotoxic when tested against HepG2 (liver hepatocellular carcinoma) cell lines with 79.96 ÃÂ± 0.67 percent cell viability at 100 mcg/mL. (positive control: 23.43 ÃÂ±0.74 percent cell viability at 100 mcg/mL).|
|Index terms / Keywords||Philippine marine sponge; Carteriospongia sp.; Cytotoxicity; Biologicallyactive compounds; Embryonic Development|