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Title Cytotoxicity of 2-Geranylgeranyl-1,4-hydroquinone Isolated from Philippine Marine Sponge Carteriospongia sp.
Posted by Chona Gelani
Authors Chona D. Gelani, Mylene M. Uy, Shinji Ohta, Emi Ohta
Conference 98th Annual Meeting of the Chemical Society of Japan
Abstract Bioassay-guided purification of the hexane:ethyl acetate extract of the Philippine marine sponge, Carteriospongia sp. , resulted in the isolation of a bioactive substance which inhibited the embryonic development of sea urchin (Hemicentrotus pulcherrimus) and starfish (Asterina pectinifera) at the early gastrulation stage at a minimum concentration of 6.25 mcg/mL and 12.5 mcg/mL, respectively. (+)HR-ESI-FTMS gave an [M+Na]+ peak corresponding to m/z 405.2763 which confirms the structure with the molecular weight 382.2865. The 1 H and 13C NMR spectra of the bioactive compound were identical to those of 2-geranylgeranyl-1,4-hydroquinone, a known cytotoxic metabolite from a southern Australian deep-water marine sponge Fasciospongia sp. As of this writing, no study has been published yet regarding the inhibitory effects of 2-geranylgeranyl-1,4-hydroquinone on the embryonic development of sea urchin and starfish. When subjected to MTT assay, 2-geranylgeranyl-1,4-hydroquinone was found to be low cytotoxic when tested against HepG2 (liver hepatocellular carcinoma) cell lines with 79.96 ± 0.67 percent cell viability at 100 mcg/mL. (positive control: 23.43 ±0.74 percent cell viability at 100 mcg/mL).
Index terms / Keywords Philippine marine sponge; Carteriospongia sp.; Cytotoxicity; Biologicallyactive compounds; Embryonic Development