Journal
| Title | Time-Dependent Demineralization of Tilapia (Oreochromis niloticus) Bones Using Hydrochloric Acid for Extracellular Matrix Extraction Posted by Hernando Bacosa |
| Authors | Nisperos, M.J., Bacosa, H., Lumancas, G., Arellano, F., Aron, J., Baclayon, L., Bantilan, Z.C., Labares, M., Jr., Bual, R. |
| Publication date | 2023 |
| Journal | Biomimetics |
| Volume | 8 |
| Issue | 217 |
| Publisher | MDPI |
| Abstract | : Tilapia (Oreochromis niloticus) is a widely cultivated fish in tropical and subtropical regions such as the Philippines, generating substantial waste during processing, including bones that are a valuable source of extracellular matrix (ECM). However, the extraction of ECM from fish bones requires an essential step of demineralization. This study aimed to assess the efficiency of tilapia bone demineralization using 0.5 N HCl at different time durations. By evaluating the residual calcium concentration, reaction kinetics, protein content, and extracellular matrix (ECM) integrity through histological analysis, composition assessment, and thermal analysis, the effectiveness of the process was determined. Results revealed that after 1 h of demineralization, the calcium and protein contents were 1.10 ± 0.12% and 88.7 ± 0.58 µg/mL, respectively. The study found that after 6 h, the calcium content was almost completely removed, but the protein content was only 51.7 ± 1.52 µg/mL compared to 109.0 ± 1.0 µg/mL in native bone tissue. Additionally, the demineralization reaction followed second-order kinetics with an R2 value of 0.9964. Histological analysis using H&E staining revealed a gradual disappearance of the basophilic components and the emergence of lacunae, which can be a |
| Index terms / Keywords | tilapia; fish bones; demineralization; extracellular matrix; kinetics |
| URL | https://www.mdpi.com/2313-7673/8/2/217 |